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Some individuals report benefits within the first few weeks, while others require 8-12 weeks to notice substantial improvements

2 Materials and Methods 2.1 Experimental Design and Sample Collection The experiment was performed at the experimental station of the Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), Braunschweig, Germany in accordance with the German Animal Welfare Act concerning the protection of experimental animals and was approved by Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Oldenburg, Germany

Preparation of DTNB working solution: Dilute the DTNB stock solution 100-fold with 0.5 mol/L, pH 8.0 Tris-HCl buffer to prepare the DTNB working solution